0.42 g HA4 and 0.46 g HA6 were obtained from 1 g HA with 16,000 U/mL LHase.
Accurate control of the proportion of HA4 and HA6 was achieved.
Highly pure HA4 and HA6 were prepared by ion exchange Q-Sepharose Fast Flow.
HA4 and HA6 from LHase showed high activity in promotion of angiogenesis.