Ferromagnetic particles as a rapid and robust sample preparation for the absolute quantification of seven eicosanoids in human plasma by UHPLC-MS/MS
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- 作者：Anna Catharina Suhr ; 1 ; anna.suhr@med.uni-muenchen.de" class="auth_mail" title="E-mail the corresponding author ; Mathias Bruegel1 ; Barbara Maier ; Lesca Miriam Holdt ; Alisa Kleinhempel ; Daniel Teupser ; Stefanie H. Grimm ; Michael Vogeser
- 关键词：Magnetic particles ; Eicosanoids ; Sample preparation ; Liquid chromatography ; Mass spectrometry ; Validation
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：1 June 2016
- 年：2016
- 卷：1022
- 期：Complete
- 页码：173-182
- 全文大小：1574 K
文摘
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Use of ferromagnetic particles as novel deproteination technique.
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Combination of magnetic particles with on-line SPE for high through-put capability.
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UHPLC–MS/MS method for absolute quantification of seven eicosanoids.
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Validation based on EMA guideline.
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Generic approach improving automation in LC–MS/MS.