Induction of primitive streak and mesendoderm formation in monolayer hESC culture by activation of TGF-尾 signaling pathway by Activin B

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• 作者：Amer Mahmood ; ^{ammahmood@ksu.edu.sa" class="auth_mail" title="E-mail the corresponding author} ; Abdullah Aldahmash ^{a.aldahmash@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Human embryonic stem cells ; Differentiation ; TGF-尾 pathway ; Mesendoderm ; Mesenchymal
• 刊名：Saudi Journal of Biological Sciences
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：22
• 期：6
• 页码：692-697
• 全文大小：1409 K

文摘

Human embryonic stem cells (hESCs) have the ability to differentiate into all human cells, however controlling the differentiation has always been a challenge. In the present study we have investigated the direct differentiation of hESCs on MEFs by using TGF-尾 signaling pathway activators Activin A and Activin B. Activation of the TGF-尾 pathway with Activin B in low serum highly induced primitive streak and mesendoderm formation after 24 h, which included up-regulation of SOX 17 and BRACHYURY protein and gene expression. Continuous stimulation with Activin B in 2% serum further induced mesendoderm formation by increased gene expression of Brachyury, SOX17, MEOX and FOX at the same time we found down-regulation of neuroectodermal marker genes. Further, by stimulating the mesodermal cells by BMP-2 we succeeded to induce mesenchymal like cells with high expression of mesenchymal markers including; MEOX, FOX, RUNX2, COL1 and OSTEOPONTIN. In conclusion we have directed the differentiation of hESCs as monolayer to primitive streak like cells with Activin B and further into pure mesoderm and mesenchymal like cells by BMP-2.