Signal enhancement in ELISA: Biotin-streptavidin technology against gold nanoparticles

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• 作者：Thangavel Lakshmipriya ; Ph.D^a ; Subash C.B. Gopinath ; Ph.D^b ; Uda Hashim ; Ph.D^b ; Thean-Hock Tang ; Ph.D^a ; ^{tangth@amdi.usm.edu.my" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Biotin ; ELISA ; GNP ; Streptavidin ; Tuberculosis
• 刊名：Journal of Taibah University Medical Sciences
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：11
• 期：5
• 页码：432-438
• 全文大小：1431 K

文摘

The aim of this study is to compare the use of biotin-streptavidin and gold nanoparticle (GNP) technologies in enzyme linked immunosorbent assay (ELISA) techniques to improve its sensitivity and accuracy.

Methods

We evaluated two ELISA methods to improve the sensitivity and accuracy. Biotin-streptavidin technology was selected to enhance the ELISA limit of detection due to the high binding affinity of biotin-streptavidin. GNP-conjugated biomolecules were selected to improve detection by ELISA. To evaluate these two methods, the early secreted antigenic target-6 (ESAT-6) from Mycobacterium tuberculosis and the anti-ESAT-6 antibody were used.

Results

The detection limit of ESAT-6 was the same with and without GNP due to the saturation of biotin and streptavidin binding. However, higher absorbance was noticed using GNP only.

Conclusion

The proposed modified ELISA can be used to screen different types of common diseases. Additionally, this study showed how several new techniques can improve the detection and accuracy of ELISA.