Development of online assay for human neutrophil elastase (HNE) inhibition by CZE-LIF.
DMSO induces adverse HNE agglomeration as shown by microscale thermophoresis (MST).
IC50 of ursolic and oleanolic acid toward HNE accurately determined by CZE-LIF.
Comparable Ki constant determined by MST and CE which validated the developed assays.
MST and CE are complementary techniques for studying biomolecule interactions.