Human neutrophil elastase inhibition studied by capillary electrophoresis with laser induced fluorescence detection and microscale thermophoresis
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- 作者：Fayad Syntia ; Reine Nehmé ; ; reine.nehme@univ-orleans.fr" class="auth_mail" title="E-mail the corresponding author ; Bé ; rengè ; re Claude ; Philippe Morin
- 关键词：Enzyme ; Inhibition ; Laser induced fluorescence ; Microscale thermophoresis ; Transverse diffusion of laminar flow profiles
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：29 January 2016
- 年：2016
- 卷：1431
- 期：Complete
- 页码：215-223
- 全文大小：1792 K
文摘
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Development of online assay for human neutrophil elastase (HNE) inhibition by CZE-LIF.
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DMSO induces adverse HNE agglomeration as shown by microscale thermophoresis (MST).
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IC50 of ursolic and oleanolic acid toward HNE accurately determined by CZE-LIF.
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Comparable Ki constant determined by MST and CE which validated the developed assays.
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MST and CE are complementary techniques for studying biomolecule interactions.