Catalytic efficiency and thermostability improvement of Suc2 invertase through rational site-directed mutagenesis
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- 作者：Nooshin Mohandesia ; Kamahldin Haghbeena ; Kamahl@nigeb.ac.ir" class="auth_mail" title="E-mail the corresponding author ; Omid Ranaeib ; Seyed Shahriar Arabc ; Sorour Hassania
- 关键词：Saccharomyces cerevisiae ; Invertase engineering ; Pichia pastoris ; Site-direct mutagenesis
- 刊名：Enzyme and Microbial Technology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：96
- 期：Complete
- 页码：14-22
- 全文大小：2780 K
文摘
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Rational method for selecting influential amino acids on catalytic efficiency and thermostability of Suc2 invertase (SInv) was explained.
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Five novel mutants of SInv were developed via site-directed mutagenesis and subjected to physicochemical examinations.
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A novel mutant (P152 V) of SInv with enhanced catalytic efficiency was introduced.
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A novel mutant (S305V-N463 V) of SInv with increased thermostability was introduced.
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The influential impacts of Pro152 and Lys153 in accommodating sucrose in SInv active site were demonstrated.