Optical spectroscopy studies of the interaction between thiophanate methyl and human serum albumin for biosensor applications

详细信息    查看全文

• 作者：I.S. Yahia^a ; ^c ; Abdulaziz A. Al-Khedhairy^b ; Javed Musarrat^b ; ^c ; F. Yakuphanoglu^d ; ^{fyhanoglu@firat.edu.tr"" rel=""nofollow} ; ^{fyhan@hotmail.com"" rel=""nofollow}
• 关键词：Biosensor ; Human serum albumin (HSA) ; Thiophanate methyl (MT) ; Optical constants ; AFM
• 刊名：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
• 出版年：2011
• 出版时间：September 2011
• 年：2011
• 卷：79
• 期：5
• 页码：1285-1290
• 全文大小：1049 K

文摘

Optical properties of the interaction between thiophanate methyl and human serum albumin have been investigated for biosensor applications. The interaction between human serum albumin (HSA) and thiophanate methyl (MT) was investigated by UV–Vis absorption spectra and atomic force microscopy. The optical constants (refractive index, absorption index, band gap and dielectric properties) of HSA, MT and MT + HSA films were determined using absorbance, transmittance and reflectance spectra. The refractive index dispersion curve (>530 nm) exhibits the normal dispersion. The refractive index of the MT + HSA is higher than both HSA and MT alone due to the highest reflectance of the mixture of MT and HSA. This behavior is indicative of the complex formation between the MT and HSA.