Analysis of the lifetime and spatial localization of hydrogen peroxide generated in the cytosol using a reduced kinetic model
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- 作者：Joseph B. Lima ; Beijing K. Huangb ; William M. Deena ; Hadley D. Sikesa ; sikes@mit.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：ODE ; ordinary differential equation ; Prx ; peroxiredoxin ; GSH ; glutathione ; GPx ; glutathione peroxidase ; Trx ; thioredoxin ; Grx ; glutaredoxin ; Srx ; sulfiredoxin ; TR ; thioredoxin reductase ; GR ; glutathione reductase ; G6PD ; glucose-6-phosphate dehydrogenase ; NADPH/NADP+ ; nicotinamide adenine dinucleotide phosphate ; AhpC ; alkyl hydroperoxide reductase
- 刊名：Free Radical Biology and Medicine
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：89
- 期：Complete
- 页码：47-53
- 全文大小：1184 K
文摘
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We reduced a kinetic model of 22 parameters and 21 species to a single equation.
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We analyzed the fraction of peroxiredoxin oxidized by H2O2.
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We estimated length and time scales of H2O2 that is generated within the cytosol.
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We used a H2O2 consumption parameter and diffusivity to obtain scale estimates.
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Cytosolic H2O2 is consumed within ~1 ms and ~1 μM of its generation.