Induced peroxidase and cytoprotective enzyme expressions support adaptation of HUVECs to sustain subsequent H₂O₂ exposure
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- 作者：Hemang Patel ; hemang@wayne.edu" class="auth_mail" title="E-mail the corresponding author ; Juan Chen ej8568@wayne.edu" class="auth_mail" title="E-mail the corresponding author ; Mahendra Kavdia kavdia@wayne.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：O₂&minus ; ; superoxide ; H₂O₂ ; hydrogen peroxide ; ROS ; reactive oxygen species ; DHE ; dihydroethidium ; EC ; endothelial cell ; HUVEC ; human umbilical vein endothelial cell ; eNOS ; constitutive nitric oxide synthase ; SOD1 ; superoxide dismutase 1 ; SOD2 ; superoxide dismutase 2 ; NOX1 ; NAD(P)H oxidase 1 ; IKBKB ; inhibitor of kappa light polypeptide gene enhancer in B-cells ; kinase beta ; CAT ; catalase ; PRDX1 ; peroxiredoxin ; GPX1 ; glutathione peroxidase 1 ; TXNRD1 ; thioredoxin reductase 1 ; TX
- 刊名：Microvascular Research
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：103
- 期：Complete
- 页码：1-10
- 全文大小：2396 K
文摘
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H₂O₂-induced regulation in redox system was studied in ECs for 72 hrs.
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Two treatment conditions: transient and consistent H₂O₂ exposures.
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At 24 hr, H₂O₂ increased O₂−, and hyperpolarized mitochondrial membrane.
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At 48 hr, upregulation in GPX1, TXNRD1, UCP1 & HMOX1 and a decrease in O₂− levels.
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At 72 hr, a similar extent of functional & genomic regulation in HUVEC in both H₂O₂ treatments.