Enhanced targeting of mitochondrial peroxide defense by the combined use of thiosemicarbazones and inhibitors of thioredoxin reductase
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- 作者：Charles R. Myersa ; b ; cmyers@mcw.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：ANF ; auranofin ; cDDP ; cisplatin (cis-diamminedichloroplatinum(II)) ; Cys ; cysteine ; DMSO ; dimethylsulfoxide ; DMEM ; Dulbecco&rsquo ; s Modified Eagle&rsquo ; s Medium ; Dp44mT ; 2 ; 2&prime ; -Dipyridyl-N ; N-dimethylsemicarbazone ; FBS ; fetal bovine serum ; Fe ; iron ; GPx ; glutathione peroxidase ; HBSS ; Hank&rsquo ; s balanced salt solution ; L-NAME ; L-NG-Nitroarginine methyl ester hydrochloride ; NEM ; N-ethylmaleimide ; PMSF ; phenylmethylsulfonyl fluoride ; Prxs ; peroxiredoxins ; Prx1 ; peroxiredoxin-1 (cytosolic) ; Prx
- 刊名：Free Radical Biology and Medicine
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：91
- 期：Complete
- 页码：81-92
- 全文大小：3543 K
文摘
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Thiosemicarbazones selectively oxidize peroxiredoxin-3 (Prx3) in cancer cells.
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Decreased cell survival to thiosemicarbazones closely correlates with Prx3 oxidation.
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Suppression of Prx3 or thioredoxin-2, which supports Prx3, enhances cytotoxicity.
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Inhibitors of thioredoxin reductase markedly enhance triapine’s cytotoxicity.
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Targeting Prx3 with thiosemicarbazone regimens is a promising anti-tumor approach.