The thioredoxin and glutathione-dependent H2O2 consumption pathways in muscle mitochondria: Involvement in H2O2 metabolism and consequence to H2O2 efflux assays
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- 作者：Daniel Munroa ; c ; daniel.munro@umanitoba.ca" class="auth_mail" title="E-mail the corresponding author ; Sheena Banha ; Emianka Sotiria ; Nahid Tamannaa ; Jason R. Treberga ; b ; c
- 关键词：AUR ; Amplex UltraRed ; HRP ; horseradish peroxidase ; NNT ; nicotinamide nucleotide transhydrogenase ; PMF ; protonmotive force ; Trx ; thioredoxin ; CDNB ; 1-chloro-2 ; 4-dintrobenzene ; FCCP ; carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone ; 2-AAPA ; 2-acetylamino-3-[4-(2-acetylamino-2-carboxyethylsulfanylthiocarbonylamino) phenylthiocarbamoylsulfanyl] propionic acid ; BCNU ; bis-chloroethylnitrosourea
- 刊名：Free Radical Biology and Medicine
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：96
- 期：Complete
- 页码：334-346
- 全文大小：878 K
文摘
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Matrix H2O2 consumers are characterized in rat skeletal muscle mitochondria.
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Consumption of H2O2 is respiration-dependent and more rapid than its production.
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Consumption relies on the thioredoxin and glutathione-dependent pathways.
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Underestimation of up to 80% of matrix-production may occur in H2O2 efflux assay.
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The fraction of H2O2 efflux detected is influenced by respiratory substrates.