Characterization of the Catalytic Domain of Human APOBEC3B and the Critical Structural Role for a Conserved Methionine
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- 作者：Sachini U. Siriwardena1 ; ; Thisari A. Guruge1 ; Ashok S. Bhagwat1 ; 2
- 关键词：CTD ; carboxy-terminal domain ; 5mC ; 5-methylcytosine ; NTD ; amino-terminal domain ; MALDI-TOF ; matrix-assisted laser desorption ionization time-of-flight ; EDTA ; ethylenediaminetetraacetic acid
- 刊名：Journal of Molecular Biology
- 出版年：2015
- 出版时间：25 September 2015
- 年：2015
- 卷：427
- 期：19
- 页码：3042-3055
- 全文大小：1520 K
文摘
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Defines and characterizes the catalytic domain of human APOBEC3B (A3B) enzyme.
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Identifies a structural role for a conserved methionine at the junction of amino terminal and carboxy terminal domains.
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Determines kinetic constants for the catalytic domain and shows that the amino terminal domain is inactive.
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Determines the selectivity of A3B for cytosine compared to 5-methylcytosine.
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These results imply that A3B is unlikely to play a major role in mutating or demethylating 5-methylcytosines in human genome.