Corticotropin releasing factor mRNA and peptide levels are differentially regulated in the developing ovine brain
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- 作者：Keiger ; C. Jane ; O'Steen ; W. Keith ; Brewer ; Gary ; Sorci-Thomas ; Mary ; Zehnder ; Timothy J. ; et. al.
- 关键词：Ribonuclease protection assay ; Central nervous system ; Hypothalamus ; Hippocampal-amygdala complex ; Frontal cerebral cortex ; Brainstem ; Development
- 刊名：Molecular Brain Research
- 出版年：1994
- 出版时间：November, 1994
- 年：1994
- 卷：27
- 期：1
- 页码：103-110
- 全文大小：923 K
文摘
The regulation of CRF mRNA and protein in the developing ovine brain has been studied to assess the hypothesis that CRF is differentially regulated in the hypothalamus (Hypo), hippocampal-amygdala complex (H&A), frontal cerebral cortex (FCC) and brainstem (BS). We used a quantitative RNase protection assay and radioimmunoassay to determine mRNA and peptide concentrations, respectively, from the last third of gestation until term (i.e., from 95 to 142 days gestation (dg); term g align=center border=0 SRC=/images/glyphs/BQ1.GIF> 145 days). The major findings from this study are: (1) Hypothalamic CRF mRNA was increased by 2-fold in 140-142 dg fetuses compared to 128-138 and 95-123 dg fetuses; P = 0.016. (2) In the hypothalamus of 140-142 dg fetuses, there was a 2.5-fold increase in CRF mRNA derived from polyadenylation at poly(A) sites 2, 3 or 4; P = 0.005. (3) In 128-138 dg fetuses, CRF mRNA in the frontal cortex was 2-fold higher than in the other brain regions during this time period; P = 0.008. (4) CRF peptide concentrations in the Hypo were 2.5-fold higher in 140-142 dg fetuses compared to 95-106 and 128-138 dg fetuses; P = 0.007. (5) CRF peptide concentrations in the frontal cortex were 5.5-fold higher in 140-142 dg fetuses compared to fetuses at 95-106 dg; P = 0.004. (6) CRF peptide concentrations in the H&A were 5-fold higher in 140-142 dg fetuses compared to 95-106 dg fetuses; P = 0.029. The results from the present study demonstrate for the first time that CRF mRNA and peptide are differentially regulated in a region-specific manner during development.