Efficient Recombinase-Mediated Cassette Exchange in hPSCs to Study the Hepatocyte Lineage Reveals AAVS1 Locus-Mediated Transgene Inhibition
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- 作者：Laura Ordová ; s1 ; 2 ; 13 ; laura.ordovas@med.kuleuven.be" class="auth_mail" title="E-mail the corresponding author ; lordovas@unizar.es" class="auth_mail" title="E-mail the corresponding author ; Ruben Boon1 ; 2 ; Mariaelena Pistoni1 ; 2 ; Yemiao Chen1 ; 2 ; 14 ; Esther Wolfs3 ; Wenting Guo4 ; 5 ; 6 ; Rangarajan Sambathkumar1 ; 2 ; Sylwia Bobis-Wozowicz7 ; 8 ; 15 ; Nicky Helsen1 ; 2 ; Jolien Vanhove1 ; 2 ; Pieter Berckmans1 ; 2 ; Qing Cai1 ; 2 ; Kim Vanuytsel1 ; 2 ; Kristel Eggermont1 ; 2 ; Veerle Vanslembrouck1 ; 2 ; Bé ; la Z. Schmidt9 ; 10 ; Susanna Raitano1 ; 2 ; Ludo Van Den Bosch4 ; 5 ; 6 ; Yaakov Nahmias11 ; 12 ; Toni Cathomen7 ; 8 ; Tom Struys3 ; Catherine M. Verfaillie1 ; 2 ; catherine.verfaillie@med.kuleuven.be" class="auth_mail" title="E-mail the corresponding author
- 刊名：Stem Cell Reports
- 出版年：2015
- 出版时间：10 November 2015
- 年：2015
- 卷：5
- 期：5
- 页码：918-931
- 全文大小：3326 K
文摘
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RMCE using positive/negative selection allows generation of transgenic hPSC lines in 15 days
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The AAVS1 locus exerts transgene inhibition by inducing de novo DNA methylation
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AAVS1-mediated silencing appears sequence and lineage-specific in vitro, yet is not present in vivo
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AAVS1 does not meet the requirements to be considered a universal safe harbor locus in vitro