Determination and identification of hydrophilic and hydrophobic arsenic species in methanol extract of fresh cod liver by RP-HPLC with simultaneous ICP-MS and ESI-Q-TOF-MS detection
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- 作者：Uriel Arroyo-Abad ; Susanne Lischka ; Christian Piechotta ; J¨¹rgen Mattusch ; Thorsten Reemtsma
- 关键词：Arsenic species ; Arsenolipids ; Marine fish ; Cod liver ; HPLC-ICP-MS/ESI-Q-TOF-MS
- 刊名：Food Chemistry
- 出版年：2013
- 出版时间：1 December, 2013
- 年：2013
- 卷：141
- 期：3
- 页码：3093-3102
- 全文大小：658 K
文摘
The present study was focused on the determination and identification of arsenic species in methanolic extracts of cod liver. Arsenic species were fractionated and the fractions analysed by RP-HPLC-ICP-MS coupled with ESI-Q-TOF-MS. The total concentration of arsenic in the fresh cod liver was analysed by ICP-MS to be 1.53 ¡À 0.02 mg As kg?1 w.w. and the extraction recovery was ca. 100 % and the column recovery >93 % . Besides polar inorganic and methylated arsenic species (>70 % ) more hydrophobic arsenic-containing fatty acids and hydrocarbons occurred. Based on the mass spectrometric data proposals for molecular structures were elaborated for 20 of the organic As species included 10 arsenic-containing fatty acids (AsFA) and an arsenic-containing hydrocarbon (AsHC) mentioned for the first time in fresh cod liver. Arsenobetaine was found as main water-soluble arsenic compound in cod liver followed by higher molecular mass arsenic-containing fatty acids and hydrocarbons.