High Throughput Mapping of Protein-Protein Interactions: Automation of the Yeast Two-Hybrid System

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• 作者：Steve A. Richmond ; Kelly Gregan ; Julian F. Burke
• 关键词：Author Keywords ; YEAST TWO-HYBRID ; PROTEIN-PROTEIN INTERACTIONS ; MEGAMATE ; GENETIX ; DATA TRACKING ; MATING STRATEGY ; BAIT ; PREY
• 刊名：Journal of the Association for Laboratory Automation
• 出版年：2002
• 出版时间：1 June 2002
• 年：2002
• 卷：7
• 期：3
• 页码：38-40
• 全文大小：524 K

文摘

Protein-protein interactions are a vital component in both the function and regulation of virtually all biological processes. In the yeast two-hybrid system, one of the most commonly-used methods for studying protein-protein interactions, protein-protein interactions are detected by expression of a reporter gene driven by the interaction between bait and prey proteins. We report here the development of an automated system, the MegaMate, for carrying out high throughput (over 100,000 clones per day) yeast two-hybrid screens with full data tracking.