High-throughput clone screening followed by protein expression cross-check: A visual assay platform

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• 作者：Partha Pratim Bose^a ; ^{get.ppb@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; ^{ppb.mm@jcbose.ac.in" class="auth_mail" title="E-mail the corresponding author} ; Prakash Kumar^b
• 关键词：Clone screening ; Colony ; Gold nanoparticle ; Plasmon ; Visual assay
• 刊名：Analytical Biochemistry
• 出版年：2017
• 出版时间：1 January 2017
• 年：2017
• 卷：516
• 期：Complete
• 页码：48-56
• 全文大小：1601 K

文摘

In high-throughput biotechnology and structural biology, molecular cloning is an essential prerequisite for attaining high yields of recombinant protein. However, a rapid, cost-effective, easy clone screening protocol is still required to identify colonies with desired insert along with a cross check method to certify the expression of the desired protein as the end product. We report an easy, fast, sensitive and cheap visual clone screening and protein expression cross check protocol employing gold nanoparticle based plasmonic detection phenomenon. This is a non-gel, non-PCR based visual detection technique, which can be used as simultaneous high throughput clone screening followed by the determination of expression of desired protein.