Protein engineering of aldolase LbDERA for enhanced activity toward real substrates with a high-throughput screening method coupled with an aldehyde dehydrogenase

详细信息    查看全文

• 作者：Xue-Cheng Jiao ; Jiang Pan ; ^{panjiang@ecust.edu.cn} ; Xu-Dong Kong ; Jian-He Xu ; ^{jianhexu@ecust.edu.cn}
• 关键词：Aldehyde dehydrogenase ; Crystal structure ; 2-Deoxyribose-5-phosphate aldolase ; High-throughput screening ; Protein engineering ; Statin side chains
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2017
• 出版时间：1 January 2017
• 年：2017
• 卷：482
• 期：1
• 页码：159-163
• 全文大小：828 K
• 卷排序：482

文摘

A high-throughput method for screening aldolase variants was established. A variant LbDERAT29L/F163Y with 7-fold improved activity was identified. The crystal structure reveals structure changes responsible for activity improvement.