Larvicidal activities of chinaberry, neem and Bacillus thuringiensis israelensis (Bti) to an insecticide resistant population of Anopheles arabiensis from Tolay, Southwest Ethiopia

详细信息    查看全文

• 作者：Assalif Demissew¹ ; ^{assalifd@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; ^{ashifera2005@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; ^{assalif.demissew@ambou.edu.et" class="auth_mail" title="E-mail the corresponding author} ; Meshesha Balkew² ; Melaku Girma³
• 关键词：Larvicide ; Anopheles arabiensis ; Tolay ; Insecticide ; Resistance ; Botanical ; Bio-efficacy
• 刊名：Asian Pacific Journal of Tropical Biomedicine
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：6
• 期：7
• 页码：554-561
• 全文大小：387 K

文摘

To elucidate the larvicidal potency of neem, chinaberry and Bacillus thuringiensis israelensis (Bti) to larvae of Anopheles arabiensis under semi-field condition and adult susceptibility/resistance to the conventionally used insecticides in Tolay, Southwestern Ethiopia.

Methods

Wild collected 3rd and 4th stage larvae were exposed to neem, and chinaberry seed powder dissolved in water and Bti in artificial containers at three treatment levels: 0.2, 0.1 and 0.05 g/m² and controls were free of treatments. Larval and pupal mortalities were monitored daily and residual activities were determined. The experiments were replicated three times. The World Health Organization tube test for all classes of insecticides was conducted on adult Anopheles arabiensis reared from field collected larvae and pupae. Data were analyzed using STATA software version 11.

Results

In the first application, neem powder caused 88.9%, 87.9% and 79.4% larval and pupal mortality at 0.2, 0.1 and 0.05 g/m² after 4.3, 6.0 and 5.7 days, respectively. The corresponding killing effect of chinaberry was 80.3%, 62.1% and 30.3% after 7.0, 7.7 and 8.3 days respectively. Bti at all treatments killed 100% after 24 h except 2.7 days for 0.05 g/m². Adult mosquitoes were susceptible only for fenitrothion and pirimiphos-methyl with 100% mortality while resistant to deltamethrin, alpha-cypermethrin, etofenprox and dichloro-diphenyl-tricgloroethane with only 9.0%, 3.0%, 5.1% and 2.0% mortalities respectively.

Conclusions

Neem, chinaberry and Bti showed potent larvicidal and pupicidal activities. However, in the area, high level of mosquito resistance to pyrethroids and dichloro-diphenyl-tricgloroethane was seen which will pose serious challenge to vector control in the future. Therefore, using integrated approach including these botanical larvicides is warranted to manage insecticide resistance.