The compounds isolated from the aerial parts of C. odorata by bioassay-guided fractionation were investigated for their inhibitory effects on the NO production and NF-¦ÊB activity in LPS-stimulated RAW264.7 cells.
Six fatty acids (S)-coriolic acid (1), (S)-coriolic acid methyl ester (2), (S)-15,16-didehydrocoriolic acid (3), (S)-15,16-didehydrocoriolic acid methyl ester (4), linoleamide (5) and linolenamide (6) were isolated. All compounds inhibited the NO production at concentrations consistent with those required for NF-¦ÊB inhibition. Compound 2 was the most active with the IC50 values of 5.22 and 5.73 ¦ÌM. The addition of a double bond in the fatty chain decreased the inhibitory effects while the methyl esterification increased the activities.
The fatty acid components in C. odorata with NF-¦ÊB inhibitory activity could explain the anti-inflammation property of this plant in traditional medicine. This study could also contribute to the better use of C. odorata for human health care.