Synthesis and evaluation of C-5 modified 2′-deoxyuridine monophosphates as inhibitors of M. tuberculosis thymidylate synthase

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• 作者：Liudmila A. Alexandrova^a ; ^{ala2004_07@mail.ru" class="auth_mail" title="E-mail the corresponding author} ; Vladimir O. Chekhov^a ; ^b ; Eduard R. Shmalenyuk^a ; Sergey N. Kochetkov^a ; Rania Abu El-Asrar^c ; Piet Herdewijn^c
• 关键词：Thymidylate synthase ; Nucleoside 5&prime ; -monophosphates ; 2&prime ; -Deoxyuridine analogs ; Mycobacterium tuberculosis ; Inhibition
• 刊名：Bioorganic & Medicinal Chemistry
• 出版年：2015
• 出版时间：15 November 2015
• 年：2015
• 卷：23
• 期：22
• 页码：7131-7137
• 全文大小：1067 K

文摘

A series of 5′-monophosphates of 5-substituted 2′-deoxyuridine analogs, which recently demonstrated in vitro substantial suppression of two strains of Mycobacterium tuberculosis growth (virulent laboratory H37Rv and multiple resistant MS-115), has been synthesized and evaluated as potential inhibitors of M. tuberculosis thymidylate synthases: classical (ThyA) and flavin dependent thymidylate synthase (ThyX). A systematic SAR study and docking revealed 5-undecyloxymethyl-2′-deoxyuridine 5′-monophosphate 3b, displaying an IC₅₀ value against ThyX of 8.32 μM. All derivatives lack activity against the ThyA. It can be assumed that the mechanism of action of 3b may be partially associated with the inhibition of the ThyX.