The inhibitory effect of dihydroartemisinin on the growth of neuroblastoma cells

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• 作者：Ling Qi¹ ; Yang Yang¹ ; Yu-Cui Liu¹ ; Tian-Xin Zhu¹ ; Song Jin¹ ; Lin Zang¹ ; Yu-Ying Zhang¹ ; Kuang Ren² ; ^{renkuang@sina.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Dihydroartemisinin ; Neuroblastoma cells ; Cyclin D1 ; Caspase-3
• 刊名：Asian Pacific Journal of Tropical Biomedicine
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：6
• 期：4
• 页码：279-282
• 全文大小：527 K

文摘

To evaluate the inhibitory effect of dihydroartemisinin on neuroblastoma cell line SH-SY5Y, explore the possible mechanism of dihydroartemisinin against neuroblastoma cells.

Methods

The cell viability of dihydroartemisinin treated SH-SY5Y cells was examined by MTT assay and morphology of cells was observed by using inverted microscope. Cell cycle was examined with flowcytometry assay, then cyclin D1 and caspase-3 proteins expression was detected by ELISA and western blotting assay.

Results

MTT analysis results showed that cell viability significantly decreased after exposure to 0.05, 0.50, 5.00 and 50.00 μmol/L dihydroartemisinin in a dose-dependent manner, and the lower density of cells was observed in treated groups. The number of cells in sub-G1 phase was increased after treatment with different doses of dihydroartemisinin compared with the control group. The expression of cyclin D1 protein was decreased, while the expression of caspase-3 protein was increased in treated group.

Conclusions

Dihydroartemisinin could inhibit the proliferation through stopping the cell cycle and inducing the apoptosis in neuroblastoma SH-SY5Y cells.