De novo assembly and analysis of midgut transcriptome of Haemaphysalis flava and identification of genes involved in blood digestion, feeding and defending from pathogens
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- 作者：Xing-Li Xua ; b ; Tian-Yin Chenga ; hn5368@163.com" class="auth_mail" title="E-mail the corresponding author ; Hu Yangb ; Zhi-Hui Liaoa
- 关键词：Transcriptome ; Haemaphysalis flava ; RNA-Seq ; Midgut
- 刊名：Infection, Genetics and Evolution
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：38
- 期：Complete
- 页码：62-72
- 全文大小：1232 K
文摘
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Two cDNA libraries from the midgut of Haemaphysalis flava at different blood-feeding phases, were successfully constructed.
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Transcripts putatively associated with blood digestion were classified into eight large categories.
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Compared with partially engorged ticks, there were 3940 up-regulated and 1568 down-regulated genes in fully engorged ticks.
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Expression patterns of nine analyzed by qPCR showed similar to those determined using RNA-Seq.