A novel method to quantify the activity of alcohol acetyltransferase Using a SnO2-based sensor of electronic nose
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- 作者：Zhongqiu Hu ; Xiaojing Li ; Huxuan Wang ; Chen Niu ; Yahong Yuan ; Tianli Yue ; yuetl305@nwsuaf.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：AATFase ; alcohol acetyltransferase ; VOCs ; volatile organic compounds ; G/G0 ; the relative conductivity i.e. the ratio of the conductivity response of the sensors to the sample gas (G) relative to the carrier gas (G0) over time ; dG/dt ; the first-derivative of the relative conductivity to time ; (dG/dt)max ; the maximum value of the first-derivative of the relative conductivity to time ; (dG/dt)calibration max ; the calibrated (dG/dt)max ; dE/dt ; enzyme activity expression ; &minus ; (dCisoamyl alcohol/dt)
- 刊名：Food Chemistry
- 出版年：2016
- 出版时间：15 July 2016
- 年：2016
- 卷：203
- 期：Complete
- 页码：498-504
- 全文大小：1364 K
文摘
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A novel method to quantify alcohol acetyltransferase activity by electronic nose.
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SnO2-based sensor was the most sensitive to reducing gas.
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(dCisoamyl alcohol/dt)max was an eigenvalue for isoamyl alcohol concentration.
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The method is feasible for determining AATFase activity of a cider yeast strain 38.
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The method provides high accuracy with high recovery rate.