Development of an optimized cytotoxicity assay system for CYP3A4-mediated metabolic activation via modified piggyBac transposition
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- 作者：Lizhen Huanga ; c ; Shuxiang Zoua ; Jifeng Denga ; Tianming Daia ; Jingwei Jiangb ; Ying Jiaa ; Renke Daia ; Shuilin Xiea ; c ; hlzh2000518@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：CYP ; cytochrome P450 ; MTT ; 3-(4 ; 5-dimethyl-2-thiazolyl)-2 ; 5-diphenyl-2-H-tetrazolium bromide ; iPSCs ; induced pluripotent stem cells ; cDNA ; complementary DNA ; PB ; piggyBac ; PBase ; piggyBac tranposase ; BSO ; L-buthionine-(S ; R)-sulfoximine ; CMV ; cytomegalovirus promoter ; RFP ; red fluorescent protein ; PURO ; Puromycin ; DMEM ; Dulbecco's Modified Eagle Medium ; FCS ; fetal calf serum ; qRT-PCR ; quantitative real-time PCR ; GAPDH ; glyceraldehyde-3-phosphate dehydrogenase ; TBST ; Tris&ndash ; HCl buffered saline
- 刊名：Toxicology in Vitro
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：32
- 期：Complete
- 页码：132-137
- 全文大小：770 K
文摘
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HepG2 cells stably expressing CYP3A4 were generated by modified piggyBac system.
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The enzyme activity of CYP3A4 sustained stably and highly over 40 passages.
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The cell lines were more sensitive to the toxic effects of bioactive compounds.
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The cell lines could be suitable for predicting CYP3A4-mediated hepatotoxicity.