Effect of chronic administration of ethanol on the regulation of the δ-subunit of GABA_A receptors in the rat brain

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• 作者：C.R. Marutha Ravindran ; Ashok K. Mehta ; Maharaj K. Ticku
• 关键词：Chronic ethanol ; GABA_A receptors ; δ ; -Subunit ; Polypeptide levels ; Immunoprecipitation ; Radioligand binding
• 刊名：Brain Research
• 出版年：2007
• 出版时间：12 October 2007
• 年：2007
• 卷：1174
• 期：Complete
• 页码：47-52
• 全文大小：323 K

文摘

In the present study, we investigated the effect of chronic ethanol (CE) administration on the polypeptide levels of the δ-subunit of GABA_A receptors and [³H]muscimol binding to the immunoprecipitated δ-subunit-containing GABA_A receptor assemblies in the rat brain. CE administration resulted a down-regulation of polypeptide levels of the δ-subunit of GABA_A receptors in the rat cerebellum and hippocampus, whereas there were no changes in the δ-subunit polypeptide levels in the rat cerebral cortex. Further, CE administration caused a down-regulation of native δ-subunit-containing GABA_A receptor assemblies in the rat cerebellum as determined by [³H]muscimol binding to the immunoprecipitated receptor assemblies. These results indicate that the δ-subunit-containing GABA_A receptors may play a role in chronic ethanol-induced tolerance and dependence.