Studies of silicon nanoparticles uptake and biodegradation in cancer cells by Raman spectroscopy

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• 作者：Elen Tolstik ; PhD^a ; ^b ; ^{Elen.tolstik@ipht-jena.de" class="auth_mail" title="E-mail the corresponding author} ; ^{Elenatolstik@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Liubov Andreevna Osminkina ; PhD^c ; ^d ; Christian Matthä ; us ; PhD^a ; ^b ; Markus Burkhardt ; BS^a ; Konstantine Eduardovich Tsurikov ; BS^c ; Uliana Alexandrovna Natashina ; BS^c ; Victor Yurievich Timoshenko ; PhD^c ; ^d ; Rainer Heintzmann ; PhD^a ; ^b ; Juergen Popp ; PhD^a ; ^b ; Vladimir Sivakov ; PhD^a
• 关键词：Silicon nanoparticles ; Raman spectroscopy ; HR-SIM microscopy ; Biodegradation ; Cancer theranostic
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：12
• 期：7
• 页码：1931-1940
• 全文大小：1555 K

文摘

In-vitro Raman micro-spectroscopy was used for diagnostics of the processes of uptake and biodegradation of porous silicon nanoparticles (SiNPs) in breast cancer cells (MCF-7 cell line). Two types of nanoparticles, with and without photoluminescence in the visible spectral range, were investigated. The spatial distribution of photoluminescent SiNPs within the cells obtained by Raman imaging was verified by high-resolution structured-illumination optical microscopy. Nearly complete biodegradation of SiNPs inside the living cells was observed after 13 days of the incubation. The results reveal new prospects of multi-modal visualization of SiNPs inside cancer cells for theranostic applications.