Identification of genes differentially expressed during early interactions between the stem rot fungus (Sclerotium rolfsii) and peanut (Arachis hypogaea) cultivars with increasing disease resistance levels

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• 作者：Ansuya Jogi^a ; John W. Kerry^b ; Timothy B. Brenneman^a ; James H. Leebens-Mack^b ; Scott E. Gold^a ; ^c ; ^{scott.gold@ars.usda.gov" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Arachis hypogaea ; Sclerotium rolfsii ; Differential gene expression ; Real-time PCR ; PR proteins
• 刊名：Microbiological Research
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：184
• 期：Complete
• 页码：1-12
• 全文大小：642 K

文摘

Sclerotium rolfsii, a destructive soil-borne fungal pathogen causes stem rot of the cultivated peanut, Arachis hypogaea. This study aimed to identify differentially expressed genes associated with peanut resistance and fungal virulence. Four peanut cultivars (A100-32, Georgia Green, GA-07W and York) with increasing resistance levels were inoculated with a virulent S. rolfsii strain to study the early plant–pathogen interaction. 454 sequencing was performed on RNAs from infected tissue collected at 4 days post inoculation, generating 225,793 high-quality reads. Normalized read counts and fold changes were calculated and statistical analysis used to identify differentially expressed genes. Several genes identified as differential in the RNA-seq experiment were selected based on functions of interest and real-time PCR employed to corroborate their differential expression. Expanding the analysis to include all four cultivars revealed a small but interesting set of genes showing colinearity between cultivar resistance and expression levels. This study identified a set of genes possibly related to pathogen response that may be useful marker assisted selection or transgenic disease control strategies. Additionally, a set of differentially expressed genes that have not been functionally characterized in peanut or other plants and warrant additional investigation were identified.