Detection of fetal messenger ribonucleic acid in maternal blood to determine fetal RhD status as a strategy for noninvasive prenatal diagnosis

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• 作者：Raghad Al-Mufti ; Catherine Howard ; Timothy Overton ; Wolfgang Holzgreve ; Dorothee Gaenshirt ; Nicholas M. Fisk ; Phillip Bennett
• 关键词：Rh alloimmunization ; Rh blood group antigens ; polymerase chain reaction ; prenatal diagnosis
• 刊名：American Journal of Obstetrics and Gynecology
• 出版年：1998
• 出版时间：July 1998
• 年：1998
• 卷：179
• 期：1
• 页码：210-214
• 全文大小：115 K

文摘

OBJECTIVES: Our purpose was to test the hypothesis that reverse transcriptase–polymerase chain reaction for fetal messenger ribonucleic acid in maternal blood is more sensitive than polymerase chain reaction from genomic deoxyribonucleic acid in prenatal determination of fetal RhD blood type.

STUDY DESIGN: Fetal nucleated erythrocytes in peripheral blood from 35 RhD-negative women were enriched by triple-density gradient centrifugation, anti-CD71 magnetic sorted, and deoxyribonucleic acid and ribonucleic acid extracted. Sensitivities of reverse transcriptase–polymerase chain reaction and polymerase chain reaction were compared to predict definitive fetal RhD blood type determined in fetal tissues.

RESULTS: Reverse transcriptase–polymerase chain reaction was significantly more accurate (P = .03) than genomic–polymerase chain reaction in predicting fetal RhD blood type, both overall (28 of 35 vs 22 of 35) and when the fetus was RhD-positive (12 of 19 vs 6 of 19).

CONCLUSIONS: Reverse transcriptase–polymerase chain reaction is more sensitive than genomic–polymerase chain reaction in detection of fetal RhD sequences in maternal blood. (Am J Obstet Gynecol 1998;179:210-4.)