Processive DNA Unwinding by RecBCD Helicase in the Absence of Canonical Motor Translocation
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- 作者：Michael J. Simon ; Joshua E. Sokoloski ; Linxuan Hao ; Elizabeth Weiland ; Timothy M. Lohman ; lohman@biochem.wustl.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：dsDNA ; double-stranded DNA ; SF1 ; superfamily 1 ; ssDNA ; single-stranded DNA ; chi ; crossover hotspot instigator ; RP ; reversed polarity ; wt ; wild-type ; FRET ; Fӧrster resonance energy transfer
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：31 July 2016
- 年：2016
- 卷：428
- 期：15
- 页码：2997-3012
- 全文大小：1971 K
文摘
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E. coli RecBCD can unwind duplex DNA processively in the absence of ssDNA translocation by the canonical motors.
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DNA melting by RecBCD can occur separately from ssDNA translocation.
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This activity requires the RecB ATPase motor, the RecB arm, and nuclease domains, but not its nuclease activity.
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The RecBC secondary translocase activity is dependent on the RecB arm domain.
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The RecB nuclease domain regulates the helicase activity of RecBCD.
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DNA unwinding by RecBCD does not occur solely by the RecB and RecD motors pulling the duplex DNA across a pin or wedge.