Kinetics and mass spectrometric measurements of myoglobin unfolding in aqueous ionic liquid solutions
详细信息 查看全文
- 作者：Miranda C. Miller ; Sylvia L. Hanna ; Kelsey G. DeFrates ; Olivia C. Fiebig ; Timothy D. Vaden ; vadent@rowan.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Myoglobin ; Ionic liquids ; Protein unfolding
- 刊名：International Journal of Biological Macromolecules
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：85
- 期：Complete
- 页码：200-207
- 全文大小：1868 K
文摘
- •
Myoglobin unfolding kinetics were measured in the presence of aqueous ionic liquids.
- •
Some ionic liquids destabilize the protein and lower the energy barrier to unfolding.
- •
MS-based HDX experiments characterized the extent of IL-induced structural changes.
- •
Ionic liquids result in a more loose, open myoglobin tertiary structure.