Photopatternable and photoactive hydrogel for on-demand generation of hydrogen peroxide in cell culture

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• 作者：Shaun P. Garl ; Royal Y. Wang ; Vijay Krishna Raghunathan ; Kit S. Lam ; Christopher J. Murphy ; Paul Russell ; Gang Sun ; Tingrui Pan
• 关键词：Oxidative stress ; Hydrogen peroxide ; Anthraquinone ; ROS
• 刊名：Biomaterials
• 出版年：February, 2014
• 年：2014
• 卷：35
• 期：5
• 页码：1762-1770
• 全文大小：2193 K

文摘

Oxidative stress, largely mediated by reactive oxygen species (ROS), is a nearly ubiquitous component in complex biological processes such as aging and disease. Optimal in聽vitro methods used in elucidating disease mechanisms would deliver of low levels of hydrogen peroxide, emulating the in聽vivo pathological state, but current methods are limited by kinetic stability or accurate measurement of the dose administered. Here we present an in聽vitro platform that exploits anthraquinone catalysts for the photocatalytic production of hydrogen peroxide. This system can be dynamically tuned to provide constant generation of hydrogen peroxide at a desired physiologic rate over at least 14 days and is described using a kinetic model. Material characterization and stability is discussed along with a proof-of-concept in聽vitro study that assessed the viability of cells as they were oxidatively challenged over 24聽h at different ROS generation rates.