Is an increased drop-in rate appropriate with enhanced DNA profiling?

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• 作者：Corina C.G. Benschop ; ^{c.benschop@nfi.minvenj.nl" class="auth_mail" title="E-mail the corresponding author} ; Elisabeth S.M. Graaf ; Titia Sijen
• 关键词：Forensic science ; Low template DNA ; Enhanced interrogation ; Capillary electrophoresis ; Drop-in alleles
• 刊名：Forensic Science International: Genetics Supplement Series
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：5
• 期：Complete
• 页码：e71-e72
• 全文大小：548 K

文摘

With enhanced capillary electrophoresis (CE) injection the fold increase in peak heights relates to the fold increase in sensitivity. As a result, DNA profiles appear similar when the amount of DNA in the PCR was adjusted according to the sensitivity of the analysis. With enhanced CE injection, however, more drop-in alleles were observed. In (probabilistic) DNA profile interpretation, it seems opportune to account for an amount of drop-in that is in accordance with the sensitivity of the method.