New vanadium complexes with 6,6鈥?dimethyl-2,2鈥?6鈥?2鈥?terpyridine in terms of structure and biological properties

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• 作者：Marta A. Fik^a ; Adam Gorczy艅ski^a ; ^b ; Maciej Kubicki^a ; Zbigniew Hnatejko^a ; Anna Wadas^c ; Pawe艂 J. Kulesza^c ; Agnieszka Lewi艅ska^d ; Ma艂gorzata Giel-Pietraszuk^e ; Eliza Wyszko^e ; ^{wyszkoe@ibch.poznan.pl" class="auth_mail" title="E-mail the corresponding author} ; Violetta Patroniak^a ; ^b ; ^{violapat@amu.edu.pl" class="auth_mail" title="E-mail the corresponding author}
• 关键词：MTT ; 3-(4 ; 5-dimethylthiazol-2-yl)-2 ; 5-diphenyltetrazolium bromide ; HeLa ; Human cervical carcinoma cell line ; T47D ; Human breast cancer cell line ; A-549 ; Human lung cancer cell line ; GM07492 ; Human fibroblasts ; apparently healthy non-fetal tissue ; CT-DNA ; Calf Thymus DNA ; EB ; ethidium bromide ; PI ; propidium iodide ; TBAP ; Tetrabutyloammonium Perchlorate ; SCE ; saturated calomel electrode
• 刊名：Polyhedron
• 出版年：2015
• 出版时间：5 September 2015
• 年：2015
• 卷：97
• 期：Complete
• 页码：83-93
• 全文大小：2235 K

文摘

Reactions of 6,6″-dimethyl-2,2′:6′,2″-terpyridine ligand L and VX₃ (X = Cl, Br) in aerobic conditions result in formation of two new mononuclear coordination compounds: [VOLCl₂] 1 and [VO₂L]₂Br₂·3H₂O 2, as revealed by means of single crystal X-ray analyses. The final form of vanadium complex in the solid state, either oxido (V(IV)) or dioxido (V(V)), was found to be contingent on the size of applied halide, as demonstrated by direct V–X interaction for small chloride (1) or ionic interaction for bulkier bromide (2). Depending on crystallization conditions, [VOLCl₂] 1 forms polymorphic green or yellow crystals from MeCN or MeOH respectively. Compounds were characterized by elemental analysis, spectrometric (ESI-MS), electrochemical, (cyclic voltammetry) spectroscopic methods (UV–VIS, FTIR, EPR) and evaluated in terms of biological properties. Their cytotoxic activity was investigated in MTT assay and supported by absorption titration spectra suggesting the possible mechanism of action going through intercalative binding mode. On the basis of high binding constants K_bind = 4.4 × 10⁵ M⁻¹ (1) and K_bind = 1.0 × 10⁶ M⁻¹ (2) the relationship between structure of the complex and DNA interaction could be established, highlighting indirect impact of counterions on the final structure formation and thus biological activity. Flow cytometry analysis further confirmed the DNA binding based mechanism of action. What is more, compounds 1 and 2 are first reported cytotoxically active vanadium systems with 2,2′:6′,2″-terpyridine scaffolded family towards T47D cell line.