Cyclic peptides identified by phage display are competitive inhibitors of the tRNA-dependent amidotransferase of Helicobacter pylori
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- 作者：Van Hau Phama ; b ; c ; van-hau.pham.1@ulaval.ca" class="auth_mail" title="E-mail the corresponding author ; Halim Maaroufib ; Roger C. Levesqueb ; d ; Jacques Lapointea ; b ; c ; Jacques.Lapointe@bcm.ulaval.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：AdT ; tRNA-dependent amidotransferase ; GatCAB ; heterotrimeric AdT ; aaRS ; aminoacyl-tRNA synthetase ; TLC ; thin-layer chromatography
- 刊名：Peptides
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：79
- 期：Complete
- 页码：8-15
- 全文大小：1338 K
文摘
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The tRNA-dependent amidotransferase GatCAB is essential for Helicobacter pylori.
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GatCAB participates in the biosynthesis of Gln-tRNAGln and/or Asn-tRNAAsn in H. pylori.
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Several cyclic peptides identified by phage display inhibit H. pylori GatCAB.
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P10 (CMPVWKPDC) is a competitive inhibitor of GatCAB with respect to Glu-tRNAGln.
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Molecular docking suggests that Trp5 and Lys6 of P10 are important for GatCAB binding.