Comprehensive selection of reference genes for expression studies in meniscus injury using quantitative real-time PCR
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- 作者：Mariana Ferreira Leala ; b ; mariana.morf@epm.br" class="auth_mail" title="E-mail the corresponding author ; Gustavo Gonç ; alves Arliania ; Diego Costa Astura ; Carlos Eduardo Franciozia ; Pedro Debieuxa ; Carlos Vicente Andreolia ; Marí ; lia Cardoso Smithb ; Alberto de Castro Pochinia ; Benno Ejnismana ; Moises Cohena
- 关键词：ACL ; anterior cruciate ligament ; RT-qPCR ; reverse transcription-quantitative polymerase chain reaction ; MRI ; magnetic resonance imaging ; TLDA ; TaqMan Low-Density Array ; AAV ; adeno-associated virus ; Crt ; relative cycle threshold ; Ct ; cycle threshold ; SD ; standard deviation ; CV ; coefficient of variance ; RQ ; relative quantification ; Acc.SD ; accumulated standard deviation
- 刊名：Gene
- 出版年：2016
- 出版时间：10 June 2016
- 年：2016
- 卷：584
- 期：1
- 页码：60-68
- 全文大小：686 K
文摘
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Stable reference genes are necessary to a reliable expression data by quantitative PCR.
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We determined a set of reference genes for qPCR use in injured and non-injured menisci.
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NormFinder, geNorm, BestKeeper DataAssist and RefFinder software packages and comparative ΔCt. method were used.
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HPRT1 + TBP + GAPDH is the most suitable combination for the analysis of injured and non-injured menisci.