Simvastatin coating of TiO2 scaffold induces osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells
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- 作者：Helen Pullisaara ; Janne E. Reselanda ; Hå ; vard J. Haugena ; Jan E. Brinchmannb ; c ; Esben Ø ; strupa ; c ; esben.ostrup@odont.uio.no" class="auth_mail
- 关键词：hAD-MSCs ; human adipose tissue-derived mesenchymal stem cells ; TiO2 ; titanium dioxide ; SIM ; simvastatin ; OPN ; osteopontin protein ; SPP1 ; osteopontin mRNA ; OPG ; osteoprotegerin protein ; TNFRSF11B ; osteoprotegerin mRNA ; OC ; osteocalcin protein ; BGLAP ; osteocalcin mRNA ; VEGFA ; vascular endothelial growth factor A protein ; VEGFA ; vascular endothelial growth factor A mRNA ; LDH ; lactate dehydrogenase ; ALP ; alkaline phosphatase protein ; ALPL ; alkaline phosphatase mRNA ; qRT-PCR ; quantitative RT-PCR
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：25 April 2014
- 年：2014
- 卷：447
- 期：1
- 页码：139-144
- 全文大小：994 K
文摘
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Tissue engineering approach combining MSCs, simvastatin and a 3D porous scaffold.
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Induction of osteogenic differentiation in MSCs by simvastatin coated scaffolds.
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Lower concentration of simvastatin needed in 3D culture than reported for 2D culture.
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A strategy for human adipose tissue-derived MSC based bone tissue engineering.