Hyphenated LC-MALDI-ToF/ToF and LC-ESI-QToF approach in proteomic characterization of honeybee venom
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- 作者：Jan Matysiaka ; jmatysiak@ump.edu.pl" class="auth_mail" title="E-mail the corresponding author ; Joanna Hajduka ; Franz Mayerb ; Romano Hebelerb ; Zenon J. Kokota
- 关键词：Honeybee venom ; MALDI ; ESI ; Combinatorial peptide ligand library ; Shotgug
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2016
- 出版时间：20 March 2016
- 年：2016
- 卷：121
- 期：Complete
- 页码：69-76
- 全文大小：937 K
文摘
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The novel CPLL sequential fractionation of bee venom proteins was used.
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Combination of LC–MALDI–ToF/ToF & LC–ESI–QToF techniques was applied.
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In total 269 bee venom proteins were detected.
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5 predicted constituents are potentially new toxic or allergic bee venom proteins.
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The results confirmed the complementary character of MALDI & ESI ionization methods.