Proteome profiling of the growth phases of Leishmania pifanoi promastigotes in axenic culture reveals differential abundance of immunostimulatory proteins
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- 作者：Pedro J. Alcoleaa ; pjalcolea@cib.csic.es" class="auth_mail" title="E-mail the corresponding author ; pjalcolea@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Ana Alonsoa ; amalonso@cib.csic.es" class="auth_mail" title="E-mail the corresponding author ; Francisco Garcí ; a-Tabaresa ; fragata@cib.csic.es" class="auth_mail" title="E-mail the corresponding author ; Marí ; a del Carmen Menab ; mcmena@cnb.csic.es" class="auth_mail" title="E-mail the corresponding author ; Sergio Ciordiab ; sciordia@cnb.csic.es" class="auth_mail" title="E-mail the corresponding author ; Vicente Larragaa ; vlarraga@cib.csic.es" class="auth_mail" title="E-mail the corresponding author
- 关键词：Leishmania pifanoi ; Differential gene expression ; 2DE ; MALDI-TOF/TOF ; Elongation factor 1β ; Tryparedoxin peroxidase ; hsp70 ; Enolase ; Aldehyde dehydrogenase
- 刊名：Acta Tropica
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：158
- 期：Complete
- 页码：240-247
- 全文大小：1384 K
文摘
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The genome of L. pifanoi has not been sequenced and its molecular and cell biology has been scarcely studied.
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This is the first insight into the proteome of L. pifanoi..
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Despite the lack of a genome sequence, we were able to identify constantly and differentially regulated proteins by the 2DE-MALDI-TOF/TOF approach.
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Certain immunostimulatory proteins previously described are over-expressed in late-logarithmic phase or stationary phase promastigotes of L. pifanoi.