Transgenesis of Tol2-mediated seamlessly constructed BAC mammary gland expression vectors in Mus musculus
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- 作者：Yaohui Yanga ; Wenyuan Wanga ; Tian Huanga ; Weimin Ruana ; Gengsheng Caoa ; b ; gscao@henu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Glutathione peroxidase ; Bacteria artificial chromosome ; galK positive and negative selection ; Tol2-transposon ; Transgenic mice
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：20 January 2016
- 年：2016
- 卷：218
- 期：Complete
- 页码：66-72
- 全文大小：1177 K
文摘
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Using galK positive and negative screening methods, we managed to replace gene sequences in the BAC precisely.
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We deleted the first 100 kb at the 5’regulatory region carried by the BAC vectors via homologous recombination.
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Pronuclear injection efficiency of BAC transgenic mice reached 8.5% using the Tol2 transposon system.