Platelet sodium-proton exchanger and phospholipid-dependent procoagulant activity in patients with type 2 diabetes

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• 作者：Beata Telejko ; Marian Tomasiak ; Halina Stelmach ; Ida Kinalska
• 刊名：Metabolism
• 出版年：2003
• 出版时间：January 2003
• 年：2003
• 卷：52
• 期：1
• 页码：102-106
• 全文大小：88 K

文摘

Platelet Na⁺/H⁺ exchanger (NHE) activity, phospholipid-dependent thrombin generation, and platelet factor 3 (PF3) availability were measured in 83 type 2 diabetics and in 40 age- and sex-matched healthy subjects. Na⁺/H⁺ exchanger activity was significantly increased in diabetic patients in comparison to the controls ([kappa ] = 4.29 [plusmn] 0.71 [times ] 10^{[minus ]3} [times ] s^{[minus ]1}v 3.21 [plusmn] 0.64 [times ] 10^{[minus ]3} [times ] s^{[minus ]1}, P [lt ] .00001). However, there was no significant difference between subjects with ([kappa ] = 4.28 [plusmn] 0.75 [times ] 10^{[minus ]3} [times ] s^{[minus ]1}) and without ([kappa ] = 4.26 [plusmn] 0.32 [times ]10^{[minus ]3} [times ] s^{[minus ]1}) arterial hypertension, as well as between patients with normo- and microalbuminuria or overt proteinuria ([kappa ] = 4.26 [plusmn] 0.58 [times ] 10^{[minus ]3} [times ] s^{[minus ]1}, [kappa ] = 4.47 [plusmn] 0.93 [times ] 10^{[minus ]3} [times ] s^{[minus ]1} and [kappa ] = 4.07 [plusmn] 0.38 [times ]10^{[minus ]3} [times ] s^{[minus ]1}, respectively). Comparatively high NHE activity was observed in the group of patients with hemoglobin A_1c (HbA_1c) less than 7.5 % . Multiple regression analysis revealed that the factors independently related to platelet Na⁺/H⁺ exchanger activity were: total PF3 activity ([beta ] = 0.77, P = .011) and triglyceride (TG) concentration ([beta ] = 0.44, P = .039). Phospholipid-dependent thrombin generation and PF3 availability were also enhanced in all plasma fractions of diabetic patients, especially in platelet-poor plasma (PPP) and platelet-free plasma (PFP) (P [lt ] .0001 and P [lt ] .00001, respectively). There was a positive correlation between NHE activity and thrombin generation, as well as with PF3 availability in all plasma fractions. Our results suggest that enhanced platelet Na⁺/H⁺ exchanger activity associated with raised phospholipid-dependent procoagulant activity may increase the risk of vascular damage in type 2 diabetic patients.