Antiserum raised against gyrase A of Acholeplasma laidlawii reacts with phytoplasma proteins

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• 作者：Koui ; Toru ; Natsuaki ; Tomohide ; Okuda ; Seiichi
• 关键词：Gyrase A antibody ; Acholeplasma ; Phytoplasma ; Mollicute
• 刊名：FEMS Microbiology Letters
• 出版年：2002
• 出版时间：January 10, 2002
• 年：2002
• 卷：206
• 期：2
• 页码：169-174
• 全文大小：351 K

文摘

Part of the gyrase A gene (gyrA) of Acholeplasma laidlawii was cloned and incorporated directly downstream from a 6×His tag segment of the pQE expression vector. The 23-kDa fusion protein was expressed as a 6×His-tagged protein in Escherichia coli. The fusion protein was purified and used as an antigen for rabbit immunization. Western immunoblot analysis revealed that the antiserum raised against the gyrase A fragment had a specific affinity for a 108-kDa protein of A. laidlawii and cross-reacted with a 107.5-kDa protein of Acholeplasma axanthum, a 107-kDa protein of Acholeplasma granularum, and 95–97-kDa proteins of several phytoplasma-infected plants. The antiserum could also detect phytoplasmas in infected plant sap. These results demonstrate that the gyrase A protein (GyrA) of A. laidlawii shares antigenicity with the GyrA of other Acholeplasma species and also with those of phytoplasmas including some from a few groups with unrelated 16S rRNAs.