A fused gene of nucleoprotein (NP) and herpes simplex virus genes (VP22) induces highly protective immunity against different subtypes of influenza virus

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• 作者：Sukumar Saha ; Shinsuke Yoshida ; Kenji Ohba ; Kiyohiko Matsui ; Tomoko Matsuda ; Fumihiko Takeshita ; Kazunori Umeda ; Yuichi Tamura ; Kentaro Okuda ; Dennis Klinman et al.
• 关键词：Influenza nucleoprotein (NP) ; Herpes simplex virus gene protein 22 (VP22) ; DNA vaccine ; A/PR/8/34 ; HSV-1 ; CD8⁺ cells ; CD4⁺ cells ; Intercellular spreading
• 刊名：Virology
• 出版年：2006
• 出版时间：10 October 2006
• 年：2006
• 卷：354
• 期：1
• 页码：48-57
• 全文大小：527 K

文摘

We evaluated the immunogenicity and protective activity of plasmid DNA vaccines encoding the influenza virus NP gene (pNP) alone or in combination with the herpes simplex virus type 1 protein 22 gene (pVP22). Optimal immune responses were observed in BALB/c mice immunized with the combination of pVP22 plus pNP, as assessed by enzyme-linked immunosorbent assay (ELISA), enzyme-linked immunospot (ELISPOT) and intracellular cytokine staining (ICCS). These mice also showed maximal resistance following challenge with the A/PR/8/34 (H1N1) and A/Udron/72 (H3N2) strains of influenza virus. The susceptibility of immunized mice to virus infection was significantly increased following depletion of either CD4⁺ or CD8⁺ T cells. These results indicate that a plasmid DNA vaccine encoding pVP22 plus NP induces a high level of cross-protective immunity against influenza virus subtypes.