Natural regulatory T cells in isolated early responders compared with dual responders with allergic asthma

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• 作者：Takashi Kinoshita ; MD^a ; ^b ; ^&lowast ; ; Adrian Baatjes ; MSc^a ; ^&lowast ; ; Steven G. Smith ; PhD^a ; Benny Dua ; MSc^a ; Richard Watson ; BSc^a ; Tomotaka Kawayama ; MD^b ; Mark Larche ; PhD^a ; Gail M. Gauvreau ; PhD^a ; Paul M. O&rsquo ; Byrne ; MD ; FRCPI^a ; ^{obyrnep@mcmaster.ca" class="auth_mail}
• 关键词：Allergic asthma ; induced sputum ; CD4+ T cells ; late asthmatic responses ; regulatory T cells
• 刊名：Journal of Allergy and Clinical Immunology
• 出版年：March, 2014
• 年：2014
• 卷：133
• 期：3
• 页码：696-703
• 全文大小：1207 K

文摘

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Background

Natural regulatory T (Treg) cells are implicated in聽the regulation of the inflammatory response in patients with allergic asthma.

Objectives

We sought to determine changes in Treg cell numbers in the airways and peripheral blood of isolated early responder (IER) versus dual responder (DR) subjects with mild allergic asthma before and after allergen challenge.

Methods

Induced sputum was collected from 22 subjects with聽allergic asthma (10 IERs and 12 DRs) and peripheral blood collected from 8 DRs with allergic asthma at 0, 7, and聽24聽hours after allergen challenge. Treg cells were identified聽by using fluorescently labeled antibodies to CD4 and聽forkhead box protein 3 and enumerated by using flow cytometry.

Results

There was a significant increase in the percentage of sputum CD4⁺ cells 24 hours after allergen challenge in both IERs and DRs. The percentage of sputum Treg cells significantly decreased 24 hours after challenge in DRs but not IERs. This change was significantly correlated with the magnitude of the late asthmatic response. There was also a significant increase in the absolute number of sputum CD4⁺ cells and Treg cells at 24聽hours in DRs only. The ratio of the number of Treg cells to CD4⁺ cells at 24 hours was significantly smaller in DRs compared with that in IERs. None of the above changes were observed in peripheral blood.

Conclusions

DRs exhibit a diminished percentage of airway Treg cells after allergen challenge that is not observed in IERs聽and a significantly lower ratio of Treg cells to CD4⁺ cells,聽which might contribute to the development of the聽late聽asthmatic聽response.