A missense mutation of the Toll-like receptor 3 gene in a patient with influenza-associated encephalopathy

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• 作者：Fumio Hidaka ; Susumu Matsuo ; Tatsushi Muta ; Koichiro Takeshige ; Tomoyuki Mizukami and Hiroyuki Nunoi
• 关键词：Toll-like receptor ; Innate immunity ; RNA virus ; Influenza-associated encephalopathy ; Missense mutation
• 刊名：Clinical Immunology
• 出版年：2006
• 出版时间：May 2006
• 年：2006
• 卷：119
• 期：2
• 页码：188-194
• 全文大小：339 K

文摘

Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns and mediate the activation of NF-κB and the production of proinflammatory cytokines, which is critical for the innate immune system. TLR3 recognizes both double-stranded RNA and the influenza A virus. Since influenza-associated encephalopathy is frequent in Japan and East Asia and its pathological mechanism remains unknown, we analyzed several genes including TLRs and the retinoic acid inducible gene I, which could be involved in the recognition of the RNA virus. In one of three patients with influenza-associated encephalopathy, we detected a novel missense mutation (F303S) in just the TLR3 gene. This was confirmed as a loss-of-function mutant in a dose-dependent manner by NF-κB and IFN-β reporter assays using wild-type and mutant TLR3-transfected HEK293 cells. Our results imply that a mutation of the TLR3 gene could be one of the factors responsible for influenza-associated encephalopathy.