Confocal scanner laser evaluation of bactericidal effect of different antibiotic mixtures used for dental pulp regeneration: a preliminary study

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• 作者：Genta Elisa¹ ; ^{genta.elisa@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Alovisi Mario¹ ; Cuffini Anna Maria² ; Mandras Narcisa² ; Luganini Anna³ ; Roana Janira² ; Pasqualini Daminano¹ ; Scotti Nicola¹ ; Berutti Elio¹
• 关键词：antibiotics ; Trimix pulp regeneration ; confocal scanner ; Triclaritro
• 刊名：Giornale Italiano di Endodonzia
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：30
• 期：1
• 页码：41-45
• 全文大小：1037 K

文摘

Evaluate the antibacterial efficacy and depth of action into dentinal tubules of different antibiotic mixtures used for dental pulp regeneration (1-9).

Methodology

Cylindrical specimens of radicular dentine (n = 72), sterilized with ethylene oxide, have been infected with Enterococcus Faecalis (3 × 10⁷ CFU/mL) for 3 weeks (10). Specimens were randomly assigned to 3 experimental groups (n = 20) plus positive (n = 6) and negative (n = 6) controls.

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TRIMIX group (ciprofloxacin, metronidazole and minocycline)

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BIMIX group (ciprofloxacin and metronidazole)

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TRICLARITRO group (ciprofloxacin, metronidazole and clarithromycin)

Each experimental group was divided in two subgroups exposed to antibiotic paste formulations added to macrogol (MG) or ialuronic acid (HA). After 3 weeks o exposition to antibiotic formulations, all specimens were vertically fractured and analyzed with confocal laser scanning microscopy (CLSM) and viability staining (Live/Dead BacLight Viability Stain) to quantitatively evaluate the ratio of dead/live bacteria into dentine tubules (11-12). Volume ratio of red fluorescence (Dead bacteria) was calculated in three-dimensional reconstructions. Differences among groups were analyzed with Kruskall-Wallis and post-hoc Dunn's test (P < 0,05). Mean penetration depth of action was recorded and differences were analyzed with one-way ANOVA and post-hoc Bonferroni's test (P < 0,05).

Results

No significant differences regarding bactericidal effect between TRIMIX and TRICLARITRO were reported (P > 0,05). All antibiotic mixtures conveyed by HA showed a better efficacy compared with MG (P < 0,05).

TRIMIX penetration mean was higher than TRICLARITRO and BIMIX (P < 0,05), but there were no differences between HA and MG sub-groups (P > 0,05).

Conclusions

TRICLARITRO antibiotic mixture showed an effective antibacterial action deep into dentinal tubules, without the risk of tooth crown staining (10).