Amplification of 17p11.2
p12, including PMP22, TOP3A, and MAPK7, in high-grade osteosarcoma
p12, including PMP22, TOP3A, and MAPK7, in high-grade osteosarcoma
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- 作者：van Dartel ; Maaike ; Cornelissen ; Peter W.A. ; Redeker ; Sandra ; Tarkkanen ; Maija ; Knuutila ; Sakari ; et. al.
- 刊名：Cancer Genetics and Cytogenetics
- 出版年：2002
- 出版时间：December, 2002
- 年：2002
- 卷：139
- 期：2
- 页码：91-96
- 全文大小：618 K
文摘
Amplification of region 17p11.2p12 has been found in 13 % –29 % of high-grade osteosarcomas, suggesting the presence of an oncogene or oncogenes that may contribute to their development. To determine the location of these putative oncogenes, we established 17p11.2p12 amplification profiles by semiquantitative PCR, using 15 microsatellite markers and seven candidate genes in 19 high-grade osteosarcomas. Most of the tumors displayed complex amplification profiles, with frequent involvement of marker D17S2041 in 17p12 and TOP3A in 17p11.2 and, in some cases, very high-level amplification of PMP22 and MAPK7 in 17p11.2. Our findings suggest that multiple amplification targets, including PMP22, TOP3A, and MAPK7 or genes close to these candidate oncogenes, may be present in 17p11.2p12 and thus contribute to osteosarcoma tumorigenesis.
p12 has been found in 13 % –29 % of high-grade osteosarcomas, suggesting the presence of an oncogene or oncogenes that may contribute to their development. To determine the location of these putative oncogenes, we established 17p11.2p12 amplification profiles by semiquantitative PCR, using 15 microsatellite markers and seven candidate genes in 19 high-grade osteosarcomas. Most of the tumors displayed complex amplification profiles, with frequent involvement of marker D17S2041 in 17p12 and TOP3A in 17p11.2 and, in some cases, very high-level amplification of PMP22 and MAPK7 in 17p11.2. Our findings suggest that multiple amplification targets, including PMP22, TOP3A, and MAPK7 or genes close to these candidate oncogenes, may be present in 17p11.2p12 and thus contribute to osteosarcoma tumorigenesis.