Identification of the Genes Involved in the Biofilm-like Structures on Actinomyces oris K20, a Clinical Isolate from an Apical Lesion

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• 作者：Chiho Mashimo ; PhD^&lowast ; ; ^{mashimo@cc.osaka-dent.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Hiroyuki Kamitani ; DDS ; PhD^&dagger ; ; Takayuki Nambu ; PhD^&lowast ; ; Kazuyoshi Yamane ; DDS ; PhD^&lowast ; ; Takeshi Yamanaka ; DDS ; PhD^&lowast ; ; Chieko Sugimori-Shinozuka ; BE^&lowast ; ; Toshiaki Tatami ; DDS ; PhD^&lowast ; ; Junichi Inoue ; DDS ; PhD^&lowast ; ; Maki Kamei ; DDS ; PhD^&lowast ; ; Shosuke Morita ; DDS ; PhD^&dagger ; ; Kai-Poon Leung ; PhD^&Dagger ; ; Hisanori Fukushima ; DDS ; PhD^&lowast ;
• 关键词：Actinomyces oris ; apical abscess ; biofilm ; polysaccharide deacetylase ; transposon mutagenesis
• 刊名：Journal of Endodontics
• 出版年：2013
• 出版时间：January 2013
• 年：2013
• 卷：39
• 期：1
• 页码：44-48
• 全文大小：467 K

文摘

Although the production of biofilm is thought to be crucial in the pathogenesis of abscess formations caused by oral resident microorganisms, the particular mechanisms are still unknown. The aim of this study was to identify gene(s) responsible for maintaining the cell surface-associated meshwork-like structures, which are found in some biofilm-producing bacteria, in a clinical isolate of Actinomyces oris K20.

Methods

Random insertional mutagenesis by using transposon EZ-Tn5 was performed against the strain K20. Transposon insertion mutants were screened by scanning electron microscopy for the absence of cell surface-associated meshwork-like structures. The disrupted genes by the transposon insertion were determined by direct genome sequencing with the transposon-end primers.

Results

Five mutants without the meshwork-like structures were identified from 175 mutants. Sequencing of flanking regions of transposon insertion revealed that 3 mutants had a gene encoded polysaccharide deacetylase, Spo0J containing ParB-like nuclease domain, and hypothetical protein, respectively. The other 2 mutants had an insertion in a noncoding region and an unidentified region, respectively.

Conclusions

Our findings indicated that these genes might be involved in the formation of meshwork-like structures on Actinomyces oris K20.