Effect of gelatin hydrogel incorporating fibroblast growth factor 2 on human meniscal cells in an organ culture model

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• 作者：Atsushi Narita ; Masatoshi Takahara ; Toshihiko Ogino ; Shigenobu Fukushima ; Yu Kimura ; Yasuhiko Tabata
• 关键词：Meniscus ; Meniscal suture ; Organ culture ; Drug delivery system ; Fibroblast ; Growth factor 2
• 刊名：The Knee
• 出版年：2009
• 出版时间：August 2009
• 年：2009
• 卷：16
• 期：4
• 页码：285-289
• 全文大小：1826 K

文摘

Efforts to use growth factors to enhance the healing potential of the meniscus have been impeded because their half-lives are too short to maintain the biological activity. The thread was coated with gelatin hydrogel and fibroblast growth factor 2 (FGF 2) was biologically stabilized by incorporating in a gelatin hydrogel-coated thread. The purpose of this study is to investigate the effect of gelatin hydrogel-coated thread incorporating FGF 2 on human meniscal cells in an organ culture. Twenty-five menisci were cut into small pieces, and selected pieces were sutured with gelatin hydrogel-coated thread incorporating FGF 2 (FGF(+) group) or physiologic saline (FGF(−) group), followed by organ culture. The meniscal samples histologically evaluated 4, 7, and 14 days later. The cell density and the number of PCNA-positive cells for the FGF(+) group were higher than those of the FGF(−) group, while the number of TUNEL-positive cells was lower. These results suggest that FGF 2 stimulates the proliferation of meniscal cells and inhibits meniscal cell death. Gelatin hydrogel-coated threads releasable FGF 2 may be useful to promote repairing of human meniscus.