Effects of androgen receptor polyglutamine tract expansion on proliferation of NG108–15 cells

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• 作者：Nakajima ; Hideto ; Kimura ; Fumiharu ; Nakagawa ; Toshimasa ; Toshiyuki ; Ikemoto ; Furutama ; Daisuke ; et. al.
• 关键词：X-linked spinal and bulbar muscular atrophy ; Androgen receptor gene ; Polyglutamine tracts ; CAG repeat ; NG108&ndash ; 15 cells ; Cell proliferation ; Cell cycle
• 刊名：Neuroscience Letters
• 出版年：1997
• 出版时间：January 31, 1997
• 年：1997
• 卷：222
• 期：2
• 页码：83-86
• 全文大小：284 K

文摘

Expansion of the polyglutamine tracts in the androgen receptor (AR) has been recognized as a cause of X-linked spinal and bulbar muscular atrophy (SBMA). In the present study, NG108–15 cells were stably transfected with expression vectors coding for either the wild type (WT) AR gene (CAG repeat number=22) or a mutated (MT) AR gene (CAG repeat number=52). Cells proliferation and cell cycle parameters were evaluated for NG108–15-WT and NG108–15-MT cells in the presence or absence of androgen. NG108–15-WT cells demonstrated an androgen-dependent increase in cell number, while NG108–15-MT cells did not. Our results demonstrate that expansion of polyglutamine tracts in the AR may affect the proliferation and differentiation of nerve cells.